Journal: Cellular and Molecular Life Sciences: CMLS
Article Title: Gene knockout and knockin by zinc-finger nucleases: current status and perspectives
doi: 10.1007/s00018-012-1204-1
Figure Lengend Snippet: Listing of organisms treated with ZFNs against specific genes, leading either to a targeted integration (by HDR) or a gene knockout (by NHEJ), with efficiencies of ZFN targeting and OTS (off-target site) analysis
Article Snippet: Rat, embryos , Microinjection of mRNA and plasmid/Sangamo modular assembly , eGFP (hemizygous), IgM and Rab38 , – , On avg. 12 %, mosaicism , 20 OTS analyzed, no mutation , Investigate feasibility of applying designed ZFN in rat , [ 30 ].
Techniques: Gene Knockout, Knock-Out, Microinjection, Injection, Plasmid Preparation, Mutagenesis, Disruption, Modification, Transmission Assay, Selection, Transfection, Marker, Expressing, In Vivo, Coagulation, Electroporation, Clone Assay, Cotransfection, Fluorescence, Transgenic Assay